1) Go to Start > Run > type "diskpart".
A DOS window will appear

2) Then type "list volume"
you will be shown the list of your all partions that you got

3) Suppose you want to hide drive E then type "select volume 3"
Then a message will appear in same window { Volume 3 is the selected volume}

4) Now type "remove letter E"
Now a message will come { Diskpart Removed the Drive letter }

sometime it requires to reboot the computer.
Diskpart will remove the letter.

Windows XP is not having capabilt...
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